Table 1.

Mechanism of action and optimal concentration of drug inhibitors used in this study.

Drug	Effect	Optimal Concentration
Amiloride (AMD)	Inhibition of Na+/H+ exchange[26,27] (MP)	50 μg/ml
Amantadine (AMN)	Stabilization of clathrin-coated vesicles[28], increases endosomal pH [29] (CME)	100 μg/ml
Chlorpromazine (CPZ)	Disrupts assembly and disassembly of clathrin from coated pits[30] (CME)	20 μM
Genistein (GST)	Inhibits tyrosine phosphorylation preventing caveolae formation[31] (CavME)	20 μg/ml
Bafilomycin A1 (BFN)	Prevents re-acidification of synaptic vesicles inhibiting release of endosomal cargo[32,33] (CME)	5 nM
Cytochalasin D (CCN)	Inhibits actin polymerization and caveolae formation [34,35] (CavMe, Fusion)	10 μM
Azidothymidine (AZT)	Inhibits reverse transcription[36]	20 μM