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Top panel: Chinese Hamster Ovary (CHO) expressing Grb2-Venus (MW ~ 60 kDa) and FGFR2-mCherry. Middle panel: Grb2-Venus (MW ~ 60 kDa) is not retained in CHO chloride salt vesicles. Bottom panel: Grb2-Venus is retained in DTT/formaldehyde vesicles.