Figure 4. Effect of futile cycling on sensitivity to H₂O₂.

Induced cultures were treated with H₂O₂ concentrations that yield at least 90% reduction in CFUs in one strain (active or inactive cycle) within 2 hours. These concentrations were 4 mM H₂O₂ for fc_pck in MOPS 10 mM glucose medium (A), 5 mM H₂O₂ for fc_atp in MOPS 10 mM glucose medium (B), 2 mM H₂O₂ for fc_atp in EZ-rich 10 mM glucose medium (C), and 1.5 mM H₂O₂ for fc_acs in EZ-rich 10 mM glucose medium (D). These assays were performed in a wild-type MG1655 background. The p-value of the log transformed values was <0.05 by hour 2 for all cycles. Uninduced (Fig. S16) and untreated (Fig. S17) controls can be found in the Supplementary material.