Table I.

Ocular tissue hesperetin (HT) concentrations (μg/gm of tissue) obtained from 10 %w/w & 20 %w/w films 1, 3 or 6 hours post topical application. All experiments were performed in triplicate.

Formulation	Dose (mg)	Duration (h)	μg/gm of Tissue
			AH	VH	RC	IC	Cornea	Sclera
10% w/w HT film	0.8 mg	1h	4.2 ± 0.4	0.05 ± 0.03	7.8 ± 0.2	37.6 ± 6.2	14.1 ± 0.2	5.1 ± 0.9
10% w/w HT film	0.8 mg	3h	1.2 ± 0.1	0.06 ± 0.02	1.8 ± 0.5	4.2 ± 1.1	8.5 ± 0.9	14.6 ± 8.8
20% w/w HT film	1.6 mg	1h	7.2 ± 3.6	1.4 ± 0.5	23.5 ± 6.9	44.9 ± 19.7	33.5 ± 5.4	53.2 ± 5.2
20% w/w HT film	1.6 mg	3h	3.4 ± 0.9	0.16 ± 0.07	3.3 ± 0.4	7.7 ± 0.8	14.1 ± 1.1	39.8 ± 16.1
20% w/w HT film	1.6 mg	6h	ND	0.03 ± 0.02	0.38 ± 0.02	1.5 ± 0.1	ND	6.1 ± 1.6

AH - Aqueous Humor; VH - Vitreous Humor; RC - Retina-Choroid & IC - Iris Ciliary bodies.