. Author manuscript; available in PMC: 2016 Jun 1.

Published in final edited form as: J Struct Funct Genomics. 2015 Apr 9;16(2):67–80. doi: 10.1007/s10969-015-9198-1

Table 4.

Yields obtained from E. coli expression vectors

			Yield (mg)^a
			Fusion Protein		Cleaved Target Protein
Vector	Promoter/Repressor	Tag	mean	median	mean	median
pVP16	T5/lacI^q	MBP	168	143	24	9
pVP68K	T5/lacI	MBP	264	293	28	24
pVP65K	T5/lacI	TVMV/MBP	60	16	4	0
pVP91A	T5/lacI	His8	21	6	9	0

Based on 33 attempts (combining purification of 22 SeMet-labeled and 11 U-¹⁵N-labeled proteins) except for pVP91A, which was based on 22 purifications of SeMet-labeled proteins.