Table 6.
Small-scale purification results for control proteins produced from wheat germ cell-free vectors. Quantity purified is given as High, Medium, or Weak1. Where a difference between vectors is seen, the reason for the weaker rating is given in parentheses.
| # | ORF ID | MW | pEU-His | pEU-His-FV |
|---|---|---|---|---|
| 1 | GO.34382 | 10990 | Higha | High |
| 2 | GO.6042 | 11736 | High | High |
| 3 | GO.14751 | 12673 | High | High |
| 4 | GO.33810 | 13169 | High | High |
| 5 | GO.81370 | 16474 | High | Medium (Purification) |
| 6 | GO.2361 | 16543 | Medium | Weak (Expression) |
| 7 | GO.11624 | 17947 | Medium | ------(Cloning) |
| 8 | GO.91592 | 19522 | High | High |
| 9 | GO.91593 | 21232 | High | High |
| 10 | GO.605 | 24537 | High | Weak (Solubility) |
| 11 | GO.91571 | 26748 | High | High |
| 12 | GO.80048 | 33324 | High | High |
| 13 | GO.37540 | 35668 | Medium | Medium |
| 14 | GO.79368 | 35735 | High | High |
| 15 | GO.70653 | 36645 | ------(Cloning) | High |
| 16 | GO.7312 | 37140 | Medium | Weak (Purification) |
| 17 | GO.8210 | 42691 | Medium | Medium |
| 18 | GO.24674 | 43156 | High | High |
| 19 | GO.34351 | 50256 | Medium | Weak (Purification) |
| 20 | GO.74329 | 60844 | Medium | Medium |
a
“High” indicates IMAC purification of > 3 μg fusion protein per 25 μL reaction, “Medium” indicates 1-3 μg, “Weak” indicates < 1 μg.