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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Invest Dermatol. 2015 Feb 10;135(6):1581–1589. doi: 10.1038/jid.2015.40

Figure 5. Immunomodulatory effects of Pentobra on human monocytes and keratinocyte cell line stimulated with P. acnes.

Figure 5

Adherent monocytes (A–D)and HaCaT (E–H) cells were cultured (2–3 x 106/ml) with P. acnes in the presence of Pentobra (26 μM), and tobramycin (26 μM)). MCP-1 (A and E), CCL22 (B and F), IP-10 (C and G), and IL-12p40 (D and H) mRNA expression was analyzed 24 hours following P. acnes stimulation. Gene expression was normalized to the housekeeping genes GAPDH and quantified by the comparative method 2−ΔΔCT. Each panel is representative of three independent donors and experiments. Data represent mean ±SD (***p ≤ 0.001). Primers used in the study are listed in supplementary Table S1.