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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Apr 7;1848(7):1536–1544. doi: 10.1016/j.bbamem.2015.03.036

Fig. 1. Pore forming activity of the supernatants of K. kingae strain PYKK081 and the RtxA-deficient strain KKNB100.

Fig. 1

Left panel: Single channel recording of an asolectin/n-decane membrane in the presence of 100μl supernatant from strain PYKK081. The aqueous phase contained 1 M KCl, 10 mM MES, pH 6.0. The applied membrane potential was 20 mV.

Right panel: Single-channel recording of an asolectin/n-decane membrane in the presence of increasing amounts of the supernatant from strain KKNB100. The arrows indicate successive additions of 200 μl supernatants to the aqueous phase bathing the membrane. The aqueous phase contained 1 M KCl, 10 mM MES, pH 6.0. No current fluctuations were observed under these conditions