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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Apr 7;1848(7):1536–1544. doi: 10.1016/j.bbamem.2015.03.036

Fig. 2. Purification and pore forming activity of K. kingae RtxA.

Fig. 2

(A) SDS-PAGE and Western blot analysis of purified RtxA. The only band of approximately 100 kDa was detected on SDS-PAGE in the purified active sample. This protein was recognized by 10A7D7, the specific anti-RtxA antibody. (B) Single channel insertions of purified RtxA in 3 independent newly formed asolectin/n-decane membranes bathed in 1M KCl, 20 mV applied voltage and (C) Histogram summarizing the pore forming activity of the sample. The predominant single-channel conductance was 1.5 nS for 148 single-channel events.