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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Apr 7;1848(7):1536–1544. doi: 10.1016/j.bbamem.2015.03.036

Table 2.

Zero-current membrane potentials of 1% asolectin/n-decane membranes in the presence of K. kingae RtxA measured for 5-fold gradients of different salts (100 mM versus 500 mM).

Salt Vm ± SD (mV) Pcation/Panion
KCl +19.9 ± 1.49 3.49
LiCl +16.59 ± 1.49 2.76
KAc +21.03 ± 0.83 3.79

The zero-current membrane potentials (Vm) were defined as the difference between the potential at the dilute side (measuring electrode) and the potential at the concentrated side. The aqueous salt solutions were used unbuffered and had a pH around pH 6. The pH of the KAc solution was adjusted to 7.0 with acetic acid. The permeability ratio Pcation/Panion was calculated using the Goldman-Hodgkin-Katz equation [37] using the average membrane potential of at least 3 individual experiments.