Table 3.
Single channel conductance of K. kingae RtxA in presence of different NEs.
| Nonelectrolyte | r (nm) | Mr (g/mol) | χ (mS/cm) | G ± SD (nS) | F | F% |
|---|---|---|---|---|---|---|
| None | - | - | 110.3 | 1.50 ± 0.14 | - | - |
| Et. glycol | 0.26 | 62 | 57.2 | 0.80 ± 0.18 | 0.94 | 114.63 |
| Glycerol | 0.31 | 92 | 49.1 | 0.80 ± 0.08 | 0.70 | 85.37 |
| Arabinose | 0.34 | 150 | 63.7 | 0.80 ± 0.05 | 1.20 | 145.46 |
| Sorbitol | 0.39 | 182 | 57.8 | 0.90 ± 0.05 | 0.73 | 89.26 |
| PEG 200 | 0.50 | 300 | 46.1 | 0.80 ± 0.06 | 0.63 | 76.41 |
| PEG 300 | 0.60 | 360 | 45.5 | 0.90 ± 0.04 | 0.47 | 56.93 |
| PEG 400 | 0.70 | 400 | 46.4 | 0.80 ± 0.03 | 0.64 | 77.27 |
| PEG 600 | 0.80 | 600 | 54.1 | 0.90 ± 0.07 | 0.64 | 78.05 |
| PEG 1000 | 0.94 | 1000 | 49.5 | 1.40 ± 0.01 | 0.06 | 7.07 |
| PEG 2000 | 1.27 | 2000 | 56.5 | 1.50 ± 0.25 | 0.00 | 0.00 |
| PEG 3350 | 1.78 | 3350 | 56.4 | 1.70 ± 0.09 | −0.12 | −14.97 |
| PEG 6000 | 2.5 | 6000 | 50.5 | 1.5 ± 0.24 | 0.00 | 0.00 |
The single channel experiments were performed in 1 M KCl and 20% (w/v) of the corresponding NE. The hydrodynamic radius (r) and the molecular mass (Mr) of the NEs as well as the conductivity (χ) of the 1M KCl solutions containing 20% of different NEs were taken from previous publications [40–42]. The single channel conductance (G) and its standard deviation (SD) were calculated from at least 50 conductance steps. Channel filling (F) and percentage of channel filling (%F) were calculated as described in the methods section. Vm=20 mV; T=20 °C. Et. Glycol: ethylene glycol.