Table 1. Specimens investigated and analyses performed in the study.
| Code | Locality | Longitude | Latitude | Altitude m a.s.l. | Host | Sex | PCR Rbf Rbr | Symbiont detected in DGGE | Genbank Accession Code | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Insect | Rickettsia | |||||||||||||||
| 28S-D2 | COI 5’ region | COI 3’ region | ITS2 | 16S | gltA | atpA | rpmE tRNAfMet | |||||||||
| Li_IT_1 | Portici, Italy | 14°21’05” N | 40°48’55” E | 29 | E. camaldulensis | ♀ | + | Rickettsia | KP143969 | KP233972 | KP143943 | KP233904 | KP233935 | KP233920 | KP233955 | |
| Li_IT_2 | ♀ | + | Rickettsia | KP143970 | KP233973 | KP143944 | KP233905 | KP233936 | KP233921 | KP233956 | ||||||
| Li_IT_3 | ♀ | + | Rickettsia | KP143971 | KP233974 | KP143945 | KP233906 | KP233937 | KP233922 | - | ||||||
| Li_IT_4 | ♀ | + | Rickettsia | KP143972 | KP233975 | KP143946 | - | KP233938 | - | - | ||||||
| Li_IT_5 | ♀ | + | Rickettsia | KP143973 | KP233976 | KP143947 | - | KP233939 | - | - | ||||||
| Li_IT_6 | S. M. al Bagno, Italy | 17°59’49” N | 40°07’33” E | 4 | E. camaldulensis | ♀ | + | Rickettsia | - | KP233977 | - | - | - | - | - | |
| Li_IT_7 | ♀ | + | Rickettsia | - | KP233978 | - | - | - | - | - | ||||||
| Li_IT_10 | Costa Saracena Sicily, Italy | 15°07’31” N | 37°18’32” E | 22 | E. camaldulensis | ♀ | + | Rickettsia | - | KP233989 | - | KP143948 | KP233907 | KP233940 | KP233923 | - |
| Li_AR_1 | La Plata, Argentina | 57°56’20” S | 34°54’17” W | 31 | E. camaldulensis | ♀ | + | Rickettsia | KP143974 | KP233979 | KP143949 | KP233908 | KP233941 | KP233924 | KP233957 | |
| Li_AR_2* | ♀ | + | Rickettsia | KP143975 | KP233980 | KP143950 | KP233909 | KP233942 | KP233925 | KP233958 | ||||||
| Li_AR_3 | ♀ | + | Rickettsia | KP143976 | KP233981 | KP143951 | KP233910 | KP233943 | KP233926 | - | ||||||
| Li_CN_1* | Hubian, Gangzhou City, Jiangxi Province, China | 114°54’38” N | 25°53’16” E | 142 | E. globulus | ♀ | + | Rickettsia | KP143987 | KP233985 | KP143962 | KP233911 | KP233944 | KP233927 | KP233964 | |
| Li_CN_2 | ♂ | + | Rickettsia | KP143988 | KP233986 | KP143963 | KP233912 | KP233945 | KP233928 | KP233965 | ||||||
| Li_CN_3 | ♀ | + | Rickettsia | KP143989 | KP233987 | KP143964 | KP233913 | KP233946 | - | - | ||||||
| Li_CN_4 | ♀ | + | Rickettsia | KP143990 | KP233988 | KP143965 | KP233914 | KP233947 | KP233929 | - | ||||||
| Li_CN_5 | ♂ | + | Rickettsia | KP143991 | - | KP233994 | KP143966 | - | - | - | - | |||||
| Li_CN_6 | ♀ | + | - | KP143992 | KP233990 | - | KP143967 | - | - | - | - | |||||
| Li_CN_7* | ♀ | + | - | KP143993 | KP233991 | - | KP143968 | - | - | - | - | |||||
| Li_CN_8* | ♀ | + | - | KP143994 | KP233992 | - | - | - | - | - | - | |||||
| Li_CN_9* | ♀ | + | - | KP143995 | KP233993 | - | - | - | - | - | - | |||||
| Li_TK_1* | Serinyol, Antakya, Hatay Province, Turkey | 36°12’48” N | 36°22’00” E | 120 | E. camaldulensis | ♀ | + | Rickettsia | KP143977 | KP233953 | KP233966 | KP143955 | KP233918 | KP233951 | KP233933 | KP233961 |
| Li_TK_2 | ♀ | + | Rickettsia | KP143978 | KP233954 | KP233967 | KP143956 | KP233919 | KP233952 | KP233934 | KP233962 | |||||
| Li_TK_3 | ♀ | + | Rickettsia | KP143979 | - | - | KP143957 | - | - | - | - | |||||
| Li_TK_4 | ♂ | + | Rickettsia | KP143980 | - | - | KP143958 | - | - | - | KP233963 | |||||
| Li_TK_5 | ♂ | + | Rickettsia | KP143981 | - | - | KP143959 | - | - | - | - | |||||
| Li_TK_6 | ♀ | + | Rickettsia | KP143982 | - | - | KP143960 | - | - | - | - | |||||
| Li_TK_7 | ♀ | + | Rickettsia | KP143983 | - | - | KP143961 | - | - | - | - | |||||
| Li_TU_1 | Mouaden, Tunisia | 09°16’00” N | 37°09’53” E | 80 | E. camaldulensis | ♀ | + | Rickettsia | KP143984 | KP233982 | KP143952 | KP233915 | KP233948 | KP233930 | KP233959 | |
| Li_TU_2 | ♀ | + | Rickettsia | KP143985 | KP233983 | KP143953 | KP233916 | KP233949 | KP233931 | KP233960 | ||||||
| Li_TU_3 | ♀ | + | Rickettsia | KP143986 | KP233984 | KP143954 | KP233917 | KP233950 | KP233932 | - | ||||||
| Bs1 | Portici, Italy | 14°21’05” N | 40°48’55” E | 29 | Ex Bactrocera oleae on Olea europea europea | ♀ | - | - | KP233970 | KP233995 | - | - | - | - | - | |
| Am1 | Bari, Italy | 16°52’00” N | 41°07’31” E | 5 | Melilotus indicus | ♀ | - | - | KP233969 | KP233971 | KP233968 | - | - | - | - | |
Li: Leptocybe invasa; Bs: Baryscapus silvestrii; Am: Aprostocetus monacoi; +: specimen Rickettsia sp. positive in specific PCR;-: not determined.
*: destructive DNA extraction.