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. 2015 May 13;10(5):e0126794. doi: 10.1371/journal.pone.0126794

Table 2. Analysis of variance and estimates of broad-sense heritabilities (H2) of relative area under the disease progress curve (rAUDPC) and infection type (IT) scores of the recombinant inbred lines derived ted from the Druchamp × Michigan Amber cross.

Environment a Source of  rAUDPC IT
(Location, year or race) variation df MS F P df MS F P
Pullman, 2006 Line 93 1853.98 14.00 <0.0001 93 7.35 6.50 <0.0001
Replication 2 107.43 0.97 0.51 2 0.26 0.23 0.79
  Error 186 132.44     186 1.13    
H 2: 0.84 0.90
Pullman, 2010 Line 93 1410.85 5.07 <0.0001 93 7.93 27.93 <0.0001
Replication 2 197.67 0.59 0.59 2 0.51 1.78 0.17
Error 186 278.27 186 0.28
H 2: 0.67 0.83
Pullman, 2011 Line 93 1362.31 14.46 <0.0001 93 6.34 65.63 <0.0001
Replication 2 175.43 1.86 0.16 2 0.14 1.48 0.23
Error 186 94.24 186 0.10
H 2: 0.69 0.94
Mt. Vernon, 2005 Line 93 1253.49 18.87 <0.0001 93 11.20 13.65 <0.0001
Replication 2 84.08 1.27 0.28 2 0.88 1.07 0.35
  Error 186 66.43     186 0.82    
H 2: 0.82 0.80
Mt. Vernon, 2010 Line 93 620.11 36.48 <0.0001 93 3.79 6.51 <0.0001
Replication 2 17.52 1.03 0.31 2 0.47 0.81 0.37
Error 186 17.00 186 0.58
H 2: 0.92 0.70
Mt. Vernon, 2011 Line 93 925.92 23.34 <0.0001 93 6.61 24.60 <0.0001
Replication 2 39.57 1.00 0.37 2 0.17 0.63 0.22
  Error 186 39.68     186 0.27    
H 2 0.88 0.93
Greenhouse, PST-25 Line 93 1312.99 31.00 <0.0001 93 11.40 13.13 <0.0001
Replication 2 27.08 0.64 0.53 2 0.99 1.14 0.32
Error 186 42.35     186 0.87    
H 2: 0.94 0.78
Greenhouse, PST-127 Line 93 875.96 26.90 <0.0001 93 7.60 4.95 <0.0001
Replication 2 35.03 1.08 0.34 2 0.73 0.66 0.60
Error 186 32.56     186 1.14    
H 2: 0.90 0.66

a The field tests in Pullman (eastern Washington) and Mt. Vernon (western Washington) were conducted under the natural infection of Puccinia striiformis f. sp. tritici. Greenhouse tests were conducted under high-temperature cycle (10–30°C); plants were inoculated at the adult growth stage of booting with races PST-25 and PST-127 that were virulent on seedlings of Druchamp; and all growth stages of Michigan Amber were susceptible to the two races.