Table 2. Analysis of variance and estimates of broad-sense heritabilities (H2) of relative area under the disease progress curve (rAUDPC) and infection type (IT) scores of the recombinant inbred lines derived ted from the Druchamp × Michigan Amber cross.
Environment a | Source of | rAUDPC | IT | ||||||
---|---|---|---|---|---|---|---|---|---|
(Location, year or race) | variation | df | MS | F | P | df | MS | F | P |
Pullman, 2006 | Line | 93 | 1853.98 | 14.00 | <0.0001 | 93 | 7.35 | 6.50 | <0.0001 |
Replication | 2 | 107.43 | 0.97 | 0.51 | 2 | 0.26 | 0.23 | 0.79 | |
Error | 186 | 132.44 | 186 | 1.13 | |||||
H 2: | 0.84 | 0.90 | |||||||
Pullman, 2010 | Line | 93 | 1410.85 | 5.07 | <0.0001 | 93 | 7.93 | 27.93 | <0.0001 |
Replication | 2 | 197.67 | 0.59 | 0.59 | 2 | 0.51 | 1.78 | 0.17 | |
Error | 186 | 278.27 | 186 | 0.28 | |||||
H 2: | 0.67 | 0.83 | |||||||
Pullman, 2011 | Line | 93 | 1362.31 | 14.46 | <0.0001 | 93 | 6.34 | 65.63 | <0.0001 |
Replication | 2 | 175.43 | 1.86 | 0.16 | 2 | 0.14 | 1.48 | 0.23 | |
Error | 186 | 94.24 | 186 | 0.10 | |||||
H 2: | 0.69 | 0.94 | |||||||
Mt. Vernon, 2005 | Line | 93 | 1253.49 | 18.87 | <0.0001 | 93 | 11.20 | 13.65 | <0.0001 |
Replication | 2 | 84.08 | 1.27 | 0.28 | 2 | 0.88 | 1.07 | 0.35 | |
Error | 186 | 66.43 | 186 | 0.82 | |||||
H 2: | 0.82 | 0.80 | |||||||
Mt. Vernon, 2010 | Line | 93 | 620.11 | 36.48 | <0.0001 | 93 | 3.79 | 6.51 | <0.0001 |
Replication | 2 | 17.52 | 1.03 | 0.31 | 2 | 0.47 | 0.81 | 0.37 | |
Error | 186 | 17.00 | 186 | 0.58 | |||||
H 2: | 0.92 | 0.70 | |||||||
Mt. Vernon, 2011 | Line | 93 | 925.92 | 23.34 | <0.0001 | 93 | 6.61 | 24.60 | <0.0001 |
Replication | 2 | 39.57 | 1.00 | 0.37 | 2 | 0.17 | 0.63 | 0.22 | |
Error | 186 | 39.68 | 186 | 0.27 | |||||
H 2 | 0.88 | 0.93 | |||||||
Greenhouse, PST-25 | Line | 93 | 1312.99 | 31.00 | <0.0001 | 93 | 11.40 | 13.13 | <0.0001 |
Replication | 2 | 27.08 | 0.64 | 0.53 | 2 | 0.99 | 1.14 | 0.32 | |
Error | 186 | 42.35 | 186 | 0.87 | |||||
H 2: | 0.94 | 0.78 | |||||||
Greenhouse, PST-127 | Line | 93 | 875.96 | 26.90 | <0.0001 | 93 | 7.60 | 4.95 | <0.0001 |
Replication | 2 | 35.03 | 1.08 | 0.34 | 2 | 0.73 | 0.66 | 0.60 | |
Error | 186 | 32.56 | 186 | 1.14 | |||||
H 2: | 0.90 | 0.66 |
a The field tests in Pullman (eastern Washington) and Mt. Vernon (western Washington) were conducted under the natural infection of Puccinia striiformis f. sp. tritici. Greenhouse tests were conducted under high-temperature cycle (10–30°C); plants were inoculated at the adult growth stage of booting with races PST-25 and PST-127 that were virulent on seedlings of Druchamp; and all growth stages of Michigan Amber were susceptible to the two races.