Fig 7. Recovery rates of influenza virus subjected to the aerosol collection procedure.

Prepared impactor plates were spiked with 10²–10³ pfu of PN99 (green) or IN11 (orange) influenza virus and were placed at a designated environmental condition as shown on the x-axis while air was pulled through them for 15 minutes (A) or 5 minutes (B). Experiments were performed in duplicate and the percentage of recovered RNA was determined by real time RT-PCR using M gene primers and infectious virus recovery was based on plaque assays. Each shaded section represents the proportion of the total amount of input virus that was recovered.