Table 1. Replication and respiratory droplet transmission of PN99 and IN11 influenza viruses in ferrets housed at different environmental conditions.
| Inoculated a | Contacts b | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Environmental Condition (°C/%RH) | Absolute Humidity (g/m3) | Presented Dose (log10 pfu) | Peak Temp Change e (day) d | Peak % Weight Loss (day) d | Peak NW c Titer (day) d | RDT f (day) | Peak Temp Change e (day) d | Peak % Weight Loss (day) d | Peak NW c Titer (day) d | |
| PN99 | 5/70 | 4.8 | 3.8±0.10 | 1.2 (2) | 16.5 (9) | 4.7±0.30 (3) | 1/3 (3) | 0.9 (9) | 8.7 (9) | 4.4 (5) |
| 23/30 | 6.2 | 4.2±0.09 | 1.4 (2) | 15.3 (7) | 4.8±0.61 (3) | 2/3 (3) | 2.0 (5) | 8.4 (7) | 3.4±1.40 (5) | |
| 23/50 | 10.3 | 4.3±0.13 | 1.8 (2) | 9.6 (7) | 4.2±0.48 (3) | 1/3 (5) | 0.7 (3) | 5.2 (5) | 3.8 (5) | |
| 23/70 | 14.4 | 3.8±0.12 | 2.4 (2) | 11.0 (7) | 4.3±0.48 (5) | 2/3 (3) | 1.7 (5) | 7.6 (9,11) | 4.7±0.30 (3) | |
| IN11 | 5/70 | 4.8 | 5.3±0.02 | 1.9 (3) | 11.0 (9) | 5.4±0.40 (3) | 0/3 | - | - | - |
| 23/30 | 6.2 | 4.3±0.18 | 3.3 (2) | 10.7 (6) | 5.3±0.56 (1) | 3/3 (3) | 0.4 (3) | 3.7 (9) | 5.3±0.58 (3) | |
| 23/50 | 10.3 | 5.5±0.02 | 3.2 (2) | 12.2 (7) | 5.2±0.89 (5) | 0/3 | - | - | - | |
| 23/70 | 14.4 | 4.8±0.14 | 2.0 (2) | 13.4 (6) | 5.7±0.26 (1) | 2/3 (1) | 2.1 (9) | 2.1 (9) | 4.8±1.10 (7) | |
aData shown represent the mean of 3 inoculated animals.
bData shown represent infected, contact animals only.
cNW, Nasal wash titer (log10 pfu/mL) ± SD is shown.
dDay post inoculation or post contact the peak was observed is shown in parentheses.
eTemperature change over baseline (35.7–39.6°C) is shown.
fRDT, Respiratory droplet transmission frequency and the day post-exposure that virus was first detected in contact animals is shown in parentheses.