Figure 7.
Requirement for Thrb gene for T3 action on the inner cortex. A–L, Male or female mice were treated with T3 or saline from P14 to P28. Immunofluorescence analysis showed that the T3-mediated expansion of the 20αHSD-positive zone (red) in +/+ mice (A–D) was impaired in Thrb−/− (E–H) and Thrbb1/b1 mice (I–L). M, Male mice treated with T3 or saline from P21 to P35. Hematoxylin and eosin-stained paraffin sections showed that T3-mediated hypertrophy in the inner cortex was impaired in Thrb−/− mice. Tyr. Hyd., tyrosine hydroxylase (for medulla, green). DAPI (blue) marks cell nuclei. Scale bars, 50 μm.