Table 1.
Antibody Table
| Peptide/Protein Target | Name of Antibody | Manufacturer, Catalog Number | Species | Dilution |
|---|---|---|---|---|
| 20αHSD | Anti-20αHSD | Antibody Research, 591009 | Rabbit polyclonal | 1:500 |
| 3βHSD | Anti-3βHSD | TransGenic Inc, KO607 | Rabbit polyclonal | 1:500 |
| AKR1B7 | Anti-AKR1B7 | Santa Cruz, sc-27763 | Goat polyclonal | 1:200 |
| β-Galactosidase | Anti-β-galactosidase | Abcam, ab9361 | Chicken polyclonal | 1:1000 |
| BrdU | Anti-BrdU | Abcam, ab6326 | Rat monoclonal | 1:1000 |
| CYP11B2 | Anti-CYP11B2 | Gift from Dr C. Gomez-Sanchez | Rabbit polyclonal | 1:500 |
| PCNA | Anti-PCNA | Millipore, CBL407 | Mouse monoclonal | 1:1000 |
| Tyrosine hydroxylase | Anti-TH | Millipore, MAB318 | Mouse monoclonal | 1:1000 |
Abbreviation: BrdU, 5-bromo-2′-deoxyuridine.