Table 2.

PCR Primer Information

Primer, Concentration	Gene Accession Number	FW Sequence	RV Sequence	Predicted Size	Annealing Temperature, ºC	Source
Kisspeptin, 500 nM	NM_178260	TGC TGC TTC TCC TCT GT	ACC GCG ATT CCT TTT CC	132	60	(37)
PR, 200 nM	NM_008829	GGT GGG CCT TCC TAA CGA G	GAC CAC ATC AGG CTC AAT GCT	120	57	(38)
PR-B, 200 nM	NM_008829	GGT CCC CCT TGC TTG CA	CAG GAC CGA GGA AAA AGC AG	120	57	(38)
ERα, 200 nM	NM_007956	TGG GCT TAT TGA CCA ACC TAG CA	AGA ATC TCC AGC CAG GCA CAC	130	60	(73)
NKB, 200 nM	NM_009312	TCT GGA AGG ATT GCT GAA AGT G	GTA GGG AAG GGA GCC AAC AG	302	60	(41)
NeuN, 200 nM	NM_001039167	CCA GGC ACT GAG GCC AGC ACA CAG C	CTC CGT GGG GTC GGA AGG GTG G	110	62	(74)
GAPDH, 200 nM	NM_017008	GCA CAG TCA AGG CCG AGA AT	GCC TTC TCC ATG GTG GTG AA	150	57–62	(75)

Abbreviations: FW, forward; RV, reverse.