Table 1.
List of Antibodies
| Peptide/Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised (Monoclonal or Polyclonal) | Dilution Used (IF;WB) |
|---|---|---|---|---|---|
| E-cadherin | — | Purified mouse anti-E-cadherin | DB Bioscience, number 610182 | Mouse monoclonal (clone 36) | 0.25 μg/mL |
| Ki-67 | — | Purified mouse anti-human Ki-67 | DB Pharmingen, number 556003 | Mouse monoclonal (clone B56) | 2 μg/mL |
| Active-caspase 3 | — | Cleaved caspase-3 (Asp175) antibody | Cell Signaling, number 9661 | Rabbit polyclonal | 1:200 |
| PECAM-1 | — | Rat monoclonal anti-mouse endothelial cell marker CD31 (PECAM-1) | Dianova, number DIA310 | Rat monoclonal (clone SZ31) | 1:20 |
| Ezrin | aa 362–585 | Ezrin/p81/80K/cytovillin Ab-1, mouse monoclonal antibody | Thermo Scientific, number MS-661-P1 | Mouse monoclonal (clone 3C12) | 2 μg/mL |
| LAMP-1 | — | Anti-LAMP-1 1D4B antibody | Hybridoma Bank, number 1D4B | Rat monoclonal (clone 1D4B) | 1:100 |
| KDEL | — | Anti-KDEL [MAC256] antibody | Abcam, number ab50601 | Rat monoclonal (clone MAC256) | 1:300 |
| Tg | — | Monoclonal mouse anti-human thyroglobulin | Dako, number M0781 | Mouse monoclonal (clone DAK-Tg6) | 1:200; 1:1000 |
| Iodo-Tg | — | Anti-iodo-thyroglobulin antibody | Provided by Dr Ris-Stalpers | Mouse monoclonal | 1:100; 1:1000 |
| GRP78 | CT (643)GEEDTSEKDEL (654) | GRP78/BiP antibody | Thermo Scientific, number PAI-014A | Rabbit polyclonal | 2 μg/mL |
| Cathepsin D | — | Cathepsin D antibody | Santa Cruz Biotechnology, number sc-6486 | Goat polyclonal | 0.2 μg/mL |
| GAPDH | GAPDH antibody | Ambion, number AM4300 | Mouse monoclonal (clone 6C5) | 0.5 μg/mL |