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. 2015 Mar 31;156(6):2211–2221. doi: 10.1210/en.2014-1638

Figure 1.

Figure 1.

Colocalization of SNX5 and IR in hRPTCs and human and rat kidney sections and lack of coimmunoprecipitation of SNX5 and IR in hRPTCs. A, IR and SNX5 colocalized (yellow, merge images) in RPTs (white arrows) and collecting ducts (red arrow) of human kidney (×100). The inset shows a selected area under higher magnification. B, IR and SNX5 colocalized (yellow, merge images) at the brush border of RPTs (white arrows) in rat kidney (×200). IR was also expressed in subapical areas in rat kidney. The inset shows a selected area under higher magnification. C, In hRPTCs, IR colocalized (yellow, merge images) weakly with SNX5 in the basal state and increased in the perinuclear area after 15 and 30 minutes of insulin (100nM) treatment. D, SNX5 and IR did not coimmunoprecipitate in hRPTCs in the basal state or after insulin (Ins) (100nM/30 min) or D1-like receptor agonist, fenoldopam (Fen) (10−6M/30 min), treatment (Tx). WB, western blotting; X, vehicle; Ab, antibody. SNX5 Ab was used for immunoprecipitation (IP) and IR Ab was used for WB.