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. 2015 Mar 31;156(6):2211–2221. doi: 10.1210/en.2014-1638

Figure 2.

Figure 2.

IR expression in SNX5-depleted hRPTCs. A, SNX5 (47–51 kDa) band could not be detected when the SNX5 antibody was preblocked by the immunizing peptide, indicating SNX5 antibody specificity to SNX5. B and C, The protein expressions of SNX5 (B) and IR (C) were decreased in SNX5-depleted hRPTCs compared with NT and EV-transfected hRPTCs (n = 3; *, P < .05 vs others, one-way ANOVA, Student-Newman-Keuls test). D and E, SNX5 (D) and IR (E) mRNA expressions, quantified by real-time PCR (qRT-PCR), were decreased in SNX5-depleted hRPTCs (n = 6; *, P < .05 vs mock, Student's t test). F, The protein expressions of SNX5 and SNX6 in SNX5 shRNA-transfected hRPTCs and the protein expressions of D1R and D5R in DRD1 shRNA-transfected hRPTCs compared with EV-transfected hRPTCs. Ab, antibody; EV, empty vector; NT, nontransfected.