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. 2015 Mar 31;156(6):2211–2221. doi: 10.1210/en.2014-1638

Figure 3.

Figure 3.

IRS1 and PKB phosphorylation in SNX5-depleted hRPTCs. A, The expression of t-IRS1was increased but p-IRSI was decreased in SNX5 shRNA-depleted hRPTCs, resulting in a decrease in the ratio of p-IRSI to t-IRSI (n = 3; *, P < .05 vs others, one-way ANOVA, Student-Newman-Keuls test). B and C, The protein expressions of SNX5 (B) and the ratio of p-PKB to t-PKB (C) were decreased in SNX5 siRNA-depleted hRPTCs (n = 3; *, P < .05 vs mock, Student's t test). D, Basal p-PKB expression was decreased in SNX5 siRNA-depleted hRPTCs, whereas p-PKB expression was markedly increased by insulin (100nM/30 min), which was also decreased in SNX5 siRNA-depleted hRPTCs (n = 4; *, P < .05 vs mock, Student's t test; #, P < .05, vs mock+insulin, Student's t test). EV, empty vector; NT, nontransfected.