Figure 6.

Colocalization and quantification of colocalization among D₁R, SNX5, and IR in hRPTCs. A, D₁R (green), SNX5 (red), and IR (blue) colocalized (white, merge images) in hRPTCs. Insulin treatment gradually increased their colocalization at the perinuclear area, reached a maximum at 30 minutes and decreased at 60 minutes. B, The bar graph shows that the colocalization of D₁R and IR gradually increased from 5 to 30 minutes and decreased at 60 minutes with insulin treatment (n = 4; *, P < .05 vs control, one-way ANOVA, Student-Newman-Keuls test). C, The bar graph shows that the increase in colocalization of SNX5 and IR reached significance at 30 minutes and decreased at 60 minutes with insulin treatment (n = 3; *, P < .05 vs control, one-way ANOVA, Student-Newman-Keuls test).