Fig. 5.
Effect of AtThrRS-dTP(1–60) peptide fragments on in vitro import of the pF1β into mitochondria and pSSU into chloroplasts. Organelles were preincubated with 20 μg of each peptide fragment prior to the incubation with the precursors. (A) Sequences of peptide fragments derived from AtThrRS-dTP(1–60). (B) Lane 1, in vitro transcription/translated product only (10% of input). Lane 2, in vitro transcription/translated product incubated with mitochondria. Lane 3, the same as lane 2, but treated with PK after the import reaction, for detection of protected translation product inside the organelle. Lanes 4–5, 6–7, 8–9 and 10–11 are the same as lanes 2–3, but mitochondria were pre-incubated with 20 μg of each peptide fragment prior to import. (C) The same as in (B) but with chloroplasts instead of mitochondria in the reaction mixtures.