Figure 4.

Morphology of murine BMSCs (left column) and Balb 3T3 (right column) in the control and experimental cultures. Three distinct cell types of BMSCs are indicated with white arrows: large flat cells (thick arrows), smaller fibroblast-like cells (arrows with dotted shaft), and small round cells (thin arrows). Morphotypes of Balb/3T3 cells are indicated with yellow arrows: large multinucleated cells (thick arrows), fibroblast-shaped cells (thin arrows), and small round cells (arrows with dotted shafts). Cytoskeleton was stained using atto-488 phalloidin; therefore, cellular bodies are stained in green. Nuclei stained with DAPI are visible as white dots, while dead cells visualized in the reaction with propidium iodide are stained in red. Magnification 100x, scale bar = 200 μm.