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. 2015 Apr 30;2015:814896. doi: 10.1155/2015/814896

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Copyright © 2015 Agnieszka Śmieszek et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Results of immunohistochemical staining for CD44, CD105, OPN, Ki67, and caspase-3 (CASP-3). In order to recognize the nuclei location, the images of specific staining were arranged with images presenting results of DAPI staining (blue-tone images). The weaker CD44⁺, CD105⁺, OPN⁺, and Ki67⁺ reactions were noted in subcutaneous fat tissues collected from experimental group. Stronger positive reaction for caspase-3 was seen in fat biopsies derived from animals treated with metformin than in control group. Immunostaining reactions were prepared in duplicate. Magnifications are 50x and 100x; scale bar is 400 μm.