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. Author manuscript; available in PMC: 2015 May 14.
Published in final edited form as: Mol Cancer Ther. 2011 Nov 14;11(1):132–142. doi: 10.1158/1535-7163.MCT-11-0343

Fig. 3.

Fig. 3

Effects of LY294002 on apigenin-induced apoptosis in U937 cells. (A) U937 cells were pretreated with 10 μM of LY294002 for 1 h, followed by the addition of 20 μM of apigenin for 12 h and 24 h. Cells were stained with Annexin V/PI, and apoptosis was determined using flow cytometry. The values obtained from Annexin V assays represent the means ± SD for three separate experiments. **Values for cells treated with apigenin and LY294002 in combination were significantly increased as compared to apigenin alone by Student’s t-test; p < 0.01. (B–D) Total cellular extracts were subjected to Western blotting using indicated antibodies. (E) Structure of LY294002 (2-morpholin-4-yl-8-phenylchromen-4-one) (38).