Fig. 6.

In vivo anti-leukemic activity of apigenin in U937 xenografts. 18 athymic nude mice were inoculated with U937 cells (2×10⁶ cells/mouse, subcutaneously) and randomly divided into three groups (6/group) for treatment with vehicle/apigenin as described in Materials and methods. (A) Representative animals from each group as indicated with tumor. (B) Average tumor volume in vehicle control mice and mice treated with 20 and 40 mg/kg apigenin on days 10 and 20. Data are means ± SD (n =18; 6 mice/group with tumors implanted on right flank of each mouse). * or ** Values p < 0.05 or p < 0.01 significantly different compared with vehicle control by Student’s t-test. (C) Body weight changes of mice during 20 days of treatment. (D–F) Tissue sections subjected to western blotting using indicated antibodies.