Figure 2.

Spearman rank correlations between land use intensity (determined following Coors et al. 2009) and (A) absolute value of the EC₅₀ (μg L⁻¹) for carbaryl of the original populations, (B, C) the change in EC₅₀ during the selection experiment [log₂ (EC₅₀ treatment/EC₅₀ original)], and (D) the change in carbaryl tolerance between the control and the carbaryl-selected populations [log₂ (EC₅₀ carbaryl-selected/EC₅₀ control)]. Panel B shows that the loss in carbaryl tolerance in control populations compared to the original populations increases with land use intensity around the pond [log₂ (EC₅₀ control/EC₅₀ original)]; this correlation is significant. Panel C shows the change in carbaryl tolerance in carbaryl-selected populations compared to the original populations in relation to land use intensity around the pond [log₂ (EC₅₀ carbaryl-selected/EC₅₀ original)]; this correlation is not significant. Panel D shows the change in carbaryl tolerance between the control and the carbaryl-selected populations in relation to land use intensity [log₂ (EC₅₀ carbaryl-selected/EC₅₀ control)]; this relationship is marginally nonsignificant.