Table B1.
Repeat motif, primer sequences and PCR annealing temperature for Champsocephalus gunnari (C. g.) and Notothenia rossii (N. r.) microsatellite loci
| Clone | Source | Repeat motif | R primer | L primer | Annealing °C | |
|---|---|---|---|---|---|---|
| C. g. locus | ||||||
| Cgu06 | Cgu25C04b | FM878469 | (AC)9(AT)3(AC)2G(TA)2 | GATCGGCCGTAATCAAAGGAGAG | TCAGTCCAAGCCAAGATTCAAGG | 63 |
| Cgu12 | Cgu25D12 | FM878475 | (TG)23 | CCAGGCTTACCTCACCAAGCAC | TGTACCACCTGGCCATTCTGTG | 63 |
| Cgu16 | Cgu25F03 | FM878479 | (AC)8GCACGG(CA)3TG(AA)2 | CCCTCTCTTTACCCATTCCTC | CCATCATTTATGGCTGATTCC | 58 |
| Cgu18 | Cgu25H04 | FM878481 | (AC)4(AT)2(AC)5TTCT(AC)11 | TCTGCGGACTCCTCTGACAGC | TCCTCCGGAGCGAGCATAAG | 60 |
| Cgu21 | Cgu26A04 | FM878484 | (CA)7CGCACG(CA)6 | CTCTGCTGCGTCCGTCTGTC | AGCCGCCTCTCTACACACAGC | 63 |
| Cgu26 | Cgu26D11 | FM878489 | (TG)11 | TCGAGTCTCAGTTCGGCATCC | CCAGTGCTCAAAGACGCTTTCC | 63 |
| Cgu30 | Cgu26E05 | FM878493 | (TG)13CGTGCGTGCATGTGC(GT)2GC(GT)5 | AGACGAGGCTCCTCGCACAC | TGGGTCGCCTCCTCTCTCAC | 60 |
| Cgu32 | Cgu26G04 | FM878495 | (GT)15 | AGACAGCTCTGACACGCTCAGG | TATACACCCAGTCAGAGACAAAGTCACAG | 60 |
| Cgu34 | Cgu26G12b | FM878497 | (GT)17 | CGTTGCTCAGCCAGTTTCTCC | GGGCCAGTGAGGATTGTTGG | 63 |
| Cgu44 | Cgu27E07 | FM878507 | (TG)14 | TTCCTGCACTCCTGCTGCTG | CTTAAGAGCATTGACTTTCTGTGTGAGC | 63 |
| Cgu50 | Cgu27H09 | FM878513 | (AC)10 | TGGCGACACAGCTTGGTATAG | GATCCCAGTCCATGTTGCAG | 63 |
| Cgu70 | Cg4A5 | * | (CA)25 | GGTTTGCCCTCTCCCATGA | GGTTGTTACAGTGTGCCGTTTG | 61 |
| N. r. locus | ||||||
| Nro03 | Nro15D05 | FM878527 | (CA)3CT(CA)2TGCACG(CA)6 | TCCCAAGCTCTGGCAATGTG | CCAAACAACACGGGTAAGTCAGC | 63 |
| Nro11 | Nro16A11 | FM878535 | (GT)10 | GCTGGGTGGAGAGGTGGAGA | TGCATGGGTAATGACCCTGTTG | 63 |
| Nro27 | Nro17E09 | FM878551 | (CT)2(CA)11 | TTAATATGCCGCCAGCAAAGG | TCCGACAACTTCAGCGCATATC | 63 |
| Nro41 | Nro18D02 | FM878537 | (CA)10 | AAGAGCGCACCTTCCACACC | GTGCAATGCAATGTGTGACCAG | 60 |
| Nro46 | Nro18G03 | FM878570 | (C)14(AC)11 | GGAATCGATTAGCTCGGGTTTATTTG | CAACCATTAGCCAATAACCAACCAAG | 63 |
| Nro50 | Nro20B11 | FM878574 | (GATA)3TAAATA(GATA)2TA(GATA)3GA(CAGA)12 | TCATACATGTTCCAATTGCTG | TTGAACTACTACATATGGATGGATG | 56 |
| Nro66 | Nro81C01 | LN614721 | (TC)6CT(TC)4CT(TC)5TT(TC)7 | CGGCTGTGCACTGACACTGAG | GGGCTGATAGGGCAGAGCAAC | 58 |
| Nro68 | Nro81F04 | LN614723 | (GT)9 | GTCTGGCAGGTGTCGAGCAG | CTGGAGGATATCCACTGACACAGC | 60 |
| Nro69 | Nro81H06 | LN614724 | (GT)5GCT(TG)4CT(TG)4CT(TG)4CGTGC(GT)8 | TTGAGCATAGAGGTGCTGCAGTG | TGACCGCGTCTCTCCATCAG | 60 |
| Cgu69 | Cg1A5 | * | (TG)42 | TGGATGTTGGTGATGAGGAC | TCAGGATGTGATAGAGAGCACTTT | 60 |
| Cgu70 | Cg4A5 | * | (CA)25 | GGTTTGCCCTCTCCCATGA | GGTTGTTACAGTGTGCCGTTTG | 60 |
*
H. R. Wilcock, unpublished data.