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. Author manuscript; available in PMC: 2016 May 11.
Published in final edited form as: Cancer Cell. 2015 May 11;27(5):631–643. doi: 10.1016/j.ccell.2015.04.008

Figure 3. Mature hematopoietic cell lineages are altered by in vivo expression of U2AF1(S34F).

Figure 3

(A) Peripheral blood white blood cell counts of mice transplanted with transgenic donor marrow following 1, 6, and 12 months of doxycycline induction of transgene expression (n=5–12). (B) Absolute count of peripheral blood monocytes (Gr-1+, CD115+), neutrophils (Gr-1+, CD115−), B cells (B220+), and T cells (CD3e+) based on WBC and flow cytometry following 1 month doxycycline (n=9–11). (C) Mature lineage distribution of bone marrow cells by flow cytometry for monocytes, neutrophils, B cells, and T cells following 1 month doxycycline (n=9–11). (D) Detection of apoptosis in bone marrow monocytes (Gr-1+, CD115+) following 5 days of doxycycline by flow cytometry for Annexin V+ (left panel, n=9–14) and intracellular phospho-H2AX (right panel, n=8–13). All data are represented as mean (+/−SD). (*p<0.05, **p<0.01, ***p<0.001) MFI, Mean Fluorescence Intensity. See also Figure S2.