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. Author manuscript; available in PMC: 2016 May 11.
Published in final edited form as: Cancer Cell. 2015 May 11;27(5):631–643. doi: 10.1016/j.ccell.2015.04.008

Figure 6. Mutant U2AF1 alters splicing in common targets across species.

Figure 6

(A) Venn diagram of the overlap of independently discovered mutant U2AF1-induced splice junction changes by DEXSeq analysis (FDR<0.1) in 3 RNA-seq datasets: transgenic mouse CMPs, primary human hematopoietic CD34+ cells, and human AML patient samples. *Indicates multiple significant junctions detected within the gene. (B) Correlation plots and r values for junctions identified by overlap of DEXSeq datasets shown in the Venn diagram. One junction per gene is shown. (C) Log2 fold change in junction expression [U2AF1(WT) versus U2AF1(S34F)] for overlapping genes (A) and recurrently mutated genes in MDS and AML identified by Fisher’s meta-analysis (Table 1), plotted in descending order based on CMP results. U2AF1 was excluded due to an inability to differentiate endogenous vs. exogenous U2AF1(WT) transcript in CD34+ cells. See also Figure S5 and Tables S5, S6.