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Properties of primer pairs used in real-time RT-PCR
| Snap23 | Stx4 | Vamp2 | |
|---|---|---|---|
| NCBI accession number | NM_022689.2 | NM_031125.1 | NM_012663.2 |
| Forward primer | TTCCGTTTCTGTGTCCAATAG | TCAGCAGACTATGTGGAAC | CTACTTGGTCCTAAGAATCC |
| Reverse primer | TTGTGCTTTCCAGAGACTCAT | CCAAGATGAGAACAGTGACAGA | CAGAAGAGTGAAGAGTAATGG |
| Optimized annealing temperature | 52.6°C | 50.5°C | 47.0°C |
Snap23: Synaptosomal-associated protein 23; Stx4: Syntaxin 4; Vamp2: Vesicle-associated membrane protein 2
