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. 2015 May 6;15:358. doi: 10.1186/s12885-015-1361-3

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© Wang et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Subcellular localization of GMBP1 and its receptor GRP78 in SGC7901/ADR and SGC7901/VCR. (A-B): a, d, g, j: The cytoplasmic localization of internalized GRP78 (green). b, e, h, k: Nuclear staining with 4, 6-diamidino-2-phenylindole (DAPI; blue). c, f, i, l: Merged images showing the relationship between GRP78 and the nucleus. (C): The internalization of the GMBP1 peptide into SGC7901/ADR and SGC7901/VCR cells. FITC-GMBP1 bound to SGC7901/ADR and SGC7901/VCR cells exhibited higher fluorescence intensity than the negative control FITC-URP group.