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. 2015 Apr 21;16:23. doi: 10.1186/s12868-015-0163-5

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© Shin et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of GPS and GP-EX on L-DOPA-induced phosphorylation of ERK1/2 in 6-OHDA-lesioned rats. ERK1/2 phosphorylation (p-ERK1/2) was evaluated by western blotting of proteins extracted from the 6-OHDA-lesioned striatum 1 h after the final L-DOPA treatment. Immunoblot images were detected by antibodies against phospho-ERK1/2, ERK1/2 and β-actin using western blotting analysis. Values of the relative density ratios of p-ERK1/2/ERK1/2 are normalized and expressed in arbitrary units as compared with saline-treated group or L-DOPA alone-treated group. The position of molecular size markers is indicated as kDa. The results are expressed as mean ± S.E.M. for 8–10 animals/group. *P < 0.05 compared with saline-treated group; ^# P < 0.05 compared with L-DOPA alone-treated group (one-way ANOVA followed by Tukey’s test).