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. 2015 May 14;5:10293. doi: 10.1038/srep10293

Figure 2.

Figure 2

OC increases the level of keratin 18 in Eca109 cells. (A) Eca109 cells treated with different concentrations (0.1, 0.5, 1, 5 and 10 μM) of OC for 24 h were analyzed by Western blotting for keratin 18. GAPDH was used as a loading control. The statistic analysis from three independent experiments was shown in the lower panel. (*p < 0.05 vs. vehicle; Dunnett′s test). (B) Eca109 cells were treated with 10 μM OC over a certain time course, and the samples were analyzed by Western blotting for keratin 18 and GAPDH. The statistic analysis from three independent experiments was shown in the lower panel. (*p < 0.05 vs. vehicle; Dunnett′s test). (C) The relative keratin 18 mRNA levels (compared with GAPDH) were analyzed by quantitative real-time PCR. Eca109 cells treated with different concentrations (1, 5 and 10 μM) of OC for 24 h were analyzed by real-time PCR. (n = 3; *p < 0.05 vs. vehicle; Dunnett′s test). (D) Eca109 cells were treated with 10 μM OC over a certain time course, and the samples were analyzed by real-time PCR for keratin 18 and GAPDH (n = 3; *p < 0.05, **p < 0.01 vs. vehicle; Dunnett′s test). (E) Immunofluorescence analysis of Eca109 cells using an anti-keratin 18 antibody. The cells were treated with 10 μM OC for 24 h and detected with anti-keratin 18 antibody using a microscope. Scale bar = 20 μm. The enlarge images were shown in the right panel of each sample. All experiments were performed at least three independent times.