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. 2015 May 14;5:10293. doi: 10.1038/srep10293

Figure 3.

Figure 3

Keratin 18 knockdown partially eliminates the inhibitory effect of OC on metastasis. (A) Eca109 cells were transfected with scramble siRNA or individual siRNAs (No. 1 to 3) to keratin 18 for 24 h and analyzed by Western blotting. (B) Eca109 cells were transfected with keratin 18 siRNA (#2) for 24 and 48 h and analyzed by Western blotting. (C) Eca109 cells were transfected with keratin 18 siRNA (#2), and 24 h after transfection, the cells were treated with or without 10 μM OC and analyzed through a wound healing assay in 24 h. (D) The cell number from (C) in the wounded regions was counted in each group from four independent experiments. (n = 4; **p < 0.01 vs. vehicle; Student′s t-test). (E) Transwell assay. Eca109 cells were transfected with keratin 18 siRNA (#2), and 24 h after transfection, the cells were treated with or without 10 μM OC for 24 h, detected using a transwell assay and stained with crystal violet. (F) Matrigel invasion assay. Eca109 cells were transfected with keratin 18 siRNA (#2), and 24 h after transfection, the cells were treated with or without 10 μM OC for 36 h, detected using a matrigel invasion assay and stained with crystal violet. (G and H) The cell number from transwell assay (E) and matrigel invasion assay (F) was counted in each group from four independent experiments. (n = 4; **p < 0.01 vs. vehicle; Student′s t-test).