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. 2015 May 14;5:10293. doi: 10.1038/srep10293

Figure 4.

Figure 4

OC increases tubulin expression. (A) Eca109 cells treated with 10 μM OC for indicated time period were analyzed by Western blotting for α-tubulin, β-tubulin, β-actin and GAPDH. (B) Eca109 cells were treated with vehicle or 10 μM OC for 24 h. The total α-tubulin, soluble α-tubulin, and polymerized α-tubulin were separated and analyzed by Western blotting. (C) The statistic analysis of the proportion of polymerized and soluble α-tubulin from (B). (n = 3; **p < 0.01 vs. vehicle; Student′s t-test). (D) Eca109 cells were transfected with YFP-tubulin plasmid. After 24 h of incubation, the cells were treated with or without 10 μM OC for 24 h, and images were obtained using a microscope. Scale bar = 20 μm. (E) Keratin 18 suppression increases tubulin expression. Eca109 cells were transfected with keratin 18 siRNA (#2) for 24 h and treated with 10 μM OC for 24 h, and the samples were analyzed by Western blotting for keratin 18, α-tubulin, β-tubulin and GAPDH.