Figure 3.

Expression of selected genes in the SGBS adipocytes co-cultured with the MCF7 cells. Differentiated SGBS cells were co-cultured with MCF7 cells for 24 h as described in Materials and methods. The mRNA levels of HIF1α, LOX1, TGF-β, and SDF1 from the co-cultured SGBS and control SGBS cells were determined by RT-PCR. The data were normalized with 18S RNA level and analyzed with the 2^−ΔΔCT method, ^*P<0.05.