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Effects of SGBS adipocytes on EMT-inducing gene expression in MCF7 cells. SGBS or SGBS transfected with siHIF1α were co-cultured with MCF7 cells. Gene expression levels of FOXC2, TWIST1, CHD1 and CHD2 were determined in the co-cultured MCF7 cells using real-time RT-PCR. The data were normalized with the 18S RNA level and analyzed with the 2−ΔΔCT method. *P<0.05 compared to the control MCF7 cells.
