Figure 1.

Mutant allele enrichment by Eprobe-PCR using primer competition. (A) Reduction of amplification of the wild-type (WT) template. The Eprobe is designed to match the WT allele sequence (WT Eprobe). WT Eprobe has greater stability to WT template than the reverse primer and reduces its amplification by competing with the reverse primer, resulting in limited amplification of the WT gene. (B) WT Eprobe has less stability to mutant type (MT) template and does not reduce its amplification. (A and B) These combinations enrich the reaction in MT amplicons.