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. 2015 Mar 30;33(6):2719–2727. doi: 10.3892/or.2015.3883

Figure 4.

Figure 4

Melting curve of each WT and mutant template using G13D mutation type of Eprobe. Full complementary primer-DNA binding yields peaks in the higher annealing temperature region (Tm=60°C). In comparison, reactions containing other mutants or WT template show mismatch peaks with lower Tm values (50°C). WT, wild-type.