Table I.
Eprobe name | Genotype | Length (bases) | Sequence |
---|---|---|---|
Rkw19d16 | Wild-type | 19 | 5′-CTCzTGCCTACGCCACCAG-3′ |
K12GCTe3 | G12A | 14 | 5′-AGCTGCTGGCGzAG-3′ |
K12TGTe3 | G12C | 14 | 5′-AGCTTGTGGCGzAG-3′ |
K12GATe3 | G12D | 14 | 5′-AGCTGATGGCGzAG-3′ |
K12CGTe3 | G12R | 14 | 5′-AGCTCGTGGCGzAG-3′ |
K12AGTe3 | G12S | 14 | 5′-AGCTAGTGGCGzAG-3′ |
K12GTTe3 | G12V | 14 | 5′-AGCTGTTGGCGzAG-3′ |
K13GACe3 | G13D | 14 | 5′-AGCTGGTGACGzAG-3′ |
Z, Dye labeling position; underlined, mutation position; bold, KRAS codon 12 and 13 region. KRAS, Kirsten rat sarcoma viral oncogene homolog.