Table 1.

List of primers used for SYBR Green quantitative PCR

Gene of interest	Primer sequence	Product length
TLR4	Forward: 5′ - ATGCATGGATCAGAAACTCAGCAA - 3′	249
	Reverse: 5′ - AAACTTCCTGGGGAAAAACTCTGG - 3′
HMGB1	Forward: 5′ - GCTCTCACAGCCATTGCAGTACAT - 3′	129
	Reverse: 5′ - AGGATCTCCTTTGCCCATGTTTAG - 3′
GAPDH	Forward: 5′ - AGGTCGGTGTGAACGGATTTG - 3′	123
	Reverse: 5′ - TGTAGACCATGTAGTTGAGGTC - 3′

TLR4, toll-like receptor 4; HMGB1, high-mobility group box 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.