Table 2.
Summary of antibodies used for immunohistochemistry
| Antibody | Manufacturer | Dilution | In combination with |
|---|---|---|---|
| Rat anti-mouse TLR4 | R & D Systems | 1:500 | GFAP, Iba-1 and ChAT |
| Rabbit anti-mouse HMGB1 | Abcam | 1:1,000 | GFAP, CD11b and ChAT |
| Mouse anti-mouse GFAP | BD Biosciences | 1:1,000 | TLR4 |
| Rabbit anti-mouse Iba-1 | Wako | 1:400 | TLR4 |
| Goat anti-mouse ChAT | Chemicon | 1:100 | TLR4 |
TLR4, toll-like receptor 4; HMGB1, high-mobility group box 1; GFAP, glial fibrillary acidic protein; ChAT, choline acetyltransferase; Iba-1, ionised calcium-binding adapter molecule-1.