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. 2015 May 14;5:9659. doi: 10.1038/srep09659

Figure 5. Gln124 protects cells more effectively against microtubule depolymerization than FL-Tau.

Figure 5

(A): Representative analysis of Tau species and total tubulin distribution in microtubule fractions, performed after 20 minutes of nocodazole treatment. (B): Quantification was performed by calculating the ratio of tubuline present in the microtubule fraction to total tubuline. The Cropped blots are displayed; Full-length blots are presented in supplementary data (as Fig. S11). (C): Confocal imaging of N15-115 cells transfected with Tau and the truncated species Met11-Tau and Gln124-Tau, and treated with nocodazole. Cells harbor bundles are designated by white arrows. Scale bar: 50 μM. (D): Quantification of cells expressing Tau, Met11-Tau or Gln124-Tau which display bundles after 20 minutes of nocodazole treatment. Error bars indicate SEM. N ≥ 3 independent experiments. ***: P ≤ 0.001. Differences between mean values were determined using One-way ANOVA followed by Fisher's LSD post hoc test.