Table 1. Final hit list.
| hit plate | well | Cosmid(s) | Primary targets | Secondary targets | Gene(s) name(s) |
N2 (wild type)
|
SV1005 (kinesin-5 mutant)
|
||
|---|---|---|---|---|---|---|---|---|---|
| Embryonic | Post-embryonic | Embryonic | Post-embryonic | ||||||
| Table summarizing the genes that genetically interact with the worm kinesin-5 and the observed developmental defects upon dsRNA treatment. Description of the abbreviations can be found at www.RNAi.org15. | |||||||||
| 1 | B08 | R06C7.10 | myo-1 | Gro | Gro | ||||
| 1 | B11 | F36H2 | F36H2.1 | tat-5 | Emb | Emb | |||
| 1 | C09 | C39E9+B0564 | C39E9.14 | dli-1 | Emb | Adl+Ste+Bag | Emb | Ste+Pvl | |
| 1 | C11 | F46A9+F30A10 | F46A9.4 | F46A9.5 | skr-2+skr-1 | Gro | Gro | ||
| 1 | D05 | W09C3+T03F1 | W09C3.6 | T03F1.5 | gsp-3+gsp-4 | Gro | Gro | ||
| 1 | D07 | W01A8 | W01A8.4 | nuo-6 | Lva | Lva | |||
| 1 | D11 | C17E4 | C17E4.5 | pabp-2 | Gro+Lva | Gro+Lva | |||
| 1 | E11 | C41G7+M01E11 | C41G7.2 | M01E11.6 | klp-16+klp-15 | Emb | Gro | Emb | Gro |
| 2 | B11 | T26E3 | T26E3.3 | par-6 | Emb | Ste | Emb | Ste | |
| 2 | E11 | T08B2 | T08B2.8 | T08B2.7 | mrpl-23+ech-1.2 | Gro | Gro | ||
| 3 | C04 | F26E4 | F26E4.8 | tba-1 | Emb | Emb | |||
| 3 | C10 | Y71F9AM | Y71F9AM.4 | Y71F9AM.7 | cogc-3+Y71F9AM.7 | Ste+Sck | Ste+Sck | ||
| 3 | F11 | F52C6+M7 | M7.1 | F52C6.12 | ubc-2(let-70)+F52C6.12 | Emb | no defect | Emb | no defect |
| 4 | E05 | F37C12 | F37C12.3 | F37C12.3 | Gro | Gro | |||
| 4 | E08 | B0285 | B0285.1 | cdtl-7 | Lva | Lva | |||
| 4 | F03 | T12G3 | T12G3.5 | mrpl-51 | Gro+Lva | Gro+Lva | |||
| 4 | H05 | B0280 | B0280.9 | B0280.9 | Gro+Ste+Sck | Gro+Ste+Sck | |||
| 5 | C11 | W09H1+several other | several his- genes | Emb | Emb | ||||
| 5 | F02 | F09E5 | F09E5.1 | pkc-3 | Emb | Emb | |||
| 5 | F08 | Y49E10+Y51H7C+ Y9D1A+Y46H3C+etc | Gro | Gro | |||||
| 6 | C03 | several | F54D5.11 | T01G5.8 | Emb | Gro+Rup | Emb | Gro+Rup+Pat | |
| 6 | C06 | F01F1 | F01F1.7 | ddx-23 | Emb | Lva | Emb | Lva | |
| 6 | C09 | F58A4 | F58A4.8 | tbg-1 | Emb | Ste+Pvl | Emb | ||
| 6 | D07 | Y43C5A | Y43C5A.6 | rad-51 | no defect | no defect | |||
| 7 | A10 | H06I04 | H06I04.3 | H06I04.3 | Gro | Gro+Ste | |||
| 7 | E09 | M04D8 | M04D8.6 | xbx-3 | no defect | ||||
| 7 | H04 | F42A8+C06C3 | C06C3.1 | mel-11 | Adl+Ste+Bag | Ste+Sck | |||
| 7 | H12 | E04A4 | E04A4.7 | cyc-2.1 | Gro | Lva | |||
| 8 | A02 | K08F11 | K08F11.4 | yars-1 | Gro | Gro+Lva | |||
| 9 | H10 | YAC Y38F2AL | Y38F2AL.4 | vha-3 | Lva | >Gro | |||
| 9 | H12 | Y6E2A | Y6E2A.9 | sfxn-1.3 | Gro+Lva | no defect | |||
| 11 | B02 | Y82E9BR | Y82E9B5.3 | Y82E9BR.3+Y82E9BR.27+Y82E9BR.30 | Gro | >Gro | |||
| 11 | B07 | F26H11 | F26H11.1 | kbp-3 (knl) | Emb | Ste | Emb | Ste+Adl+Gro+Pvl | |
| 11 | D03 | F11A3+C50F4 | F11A3.2 | F11A3.2 | Gro | Ste+Lva | |||
| 12 | H02 | T27B1 | T27B1.2 | ztf-19/pat-9 | Emb | Emb | Pat+Adl+Rup+Dpy | ||
| 12 | F04 | T27B1 | T27B1.2 | ztf-19/pat-9 | Emb | Ste+Sck | Emb | ||
| 13 | D05 | F25G6 | F25G6.2 | symk-1 | Emb | no defect | Emb | Gro+Sck+Adl | |
| 13 | H02 | F49C12 | F49C12.13 | vha-17 | Gro | ||||