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Luciferase reporter assay for the 3′UTR of CCND2 in PC-3 cells. (A) Transfection of pre-miR-375 resulted in an increase of miR-375 upon transfection. (B) Luciferase activity was measured upon 48 and 72 h upon co-transfection of control vector with miR-NC and CCND2 with pre-miR-375. Data shown as the median and range of three independent experiments performed in triplicates (***P < 0.001; **P < 0.01).
