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. Author manuscript; available in PMC: 2015 May 14.
Published in final edited form as: Aging Cell. 2008 Sep 5;7(6):866–878. doi: 10.1111/j.1474-9726.2008.00432.x

Figure 3. Reduced cellular ROS and oxidative damage in Tg(PRDX3) mice.

Figure 3

A. Levels of cellular ROS in skin fibroblasts from Tg(PRDX3) mice and Wt mice were measured by a DCF fluorescence method. The data are presented as mean ± SEM of data obtained from three independent lines. *: P< 0.05. n=5

B. Levels of F2-isoptostanes in liver tissues of Wt and Tg(PRDX3) were determined as described by Ran et al (2007). The data are expressed as mean ± SEM. *P<0.05. n=5

C. 4-HNE adducts in mitochondria proteins from liver tissues of Tg(PRDX3) mice and Wt mice were determined by Western blots and quantified as described in Methods. The mean value of 4-HNE adducts obtained from Wt mice was artificially assigned as 1, and the relative values are presented as mean ± SEM. *: P<0.05. n= 4–5.