Fig. 2. P’s IRF7 alleles are loss-of-function by different mechanisms.
(A to C) Localization of FLAG-tagged wild-type (A), F410V (B), or Q421X (C) IRF7 in uninfected or Sendai virus–infected Vero cells by immunofluorescence imaging. This result is representative of two experiments. (D) Wild-type and mutant IRF7 dimerization by immunoprecipitation (IP) with antibody to FLAG followed by Western blot with antibodies to FLAG and HA.WCL, whole-cell lysate. (E) Localization of FLAG F410V (top) and FLAG wild-type IRF7 (bottom) cotransfected with HA Q421X IRF7 in Vero cells as assessed by immunofluorescence imaging. This result is representative of two experiments.